EntiLink™ Reverse Transcriptase from ELK Biotechnology

Product Specs
ItemEntiLink™ Reverse Transcriptase
CompanyELK Biotechnology
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberEQ002
ApplicationsqPCR, RT-PCR
Quantity10000 U

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ELK Biotechnology

1312 17th Street #692

Denver, CO 80202

United States

Phone: +12127290609

Email: ELKbio@ELKbiotech.com

Company Profile

Website: http://www.elkbiotech.com/

(1) Quercetin is a Potential Therapy for Rheumatoid Arthritis via Targeting Caspase-8 Through Ferroptosis and PyroptosisJ Inflamm ResDecember 1, 2023Qingcong Zheng, Du Wang, Rongjie Lin, Yuchao Chen, Zixing Xu, Weihong XuRNA fully. (2) First-strand cDNA synthesis. The M-MLV Reverse Transcriptase kit (ELK Biotechnology, EQ002) was used. (3) RT-qPCR. Primers H-GAPDH and H-caspase-8 are shown in Table 2. A StepOne™ Real-Time PCR

(2) Adenosine 2 receptor regulates autophagy and apoptosis to alleviate ischemia reperfusion injury in type 2 diabetes via IRE-1 signalingBMC Cardiovascular DisordersMarch 24, 2023Mohamed Bassirou Yacouba Moukeila, Erick Thokerunga, Feng He, Christian Cedric Bongolo, Yun Xia, Fuyu Wang, Adamou Foumakoye Gado, Hama Mamoudou, Shahzad Khan, Bonkano Ousseina, et al.instructions. Reverse transcription to make cDNA was conducted using M-MLV Reverse Transcriptase kit (Cat: EQ002) also from ELK Biotechnology Co. Ltd., China. Quantitative real-time PCR was performed using StepOne™ Real-Time PCR system

(3) Construction of a potentially functional lncRNA-miRNA-mRNA network in sepsis by bioinformatics analysisFrontiers in GeneticsNovember 15, 2022Li-ming Zheng, Jun-qiu Ye, Heng-fei Li, Quan LiuTRIpure reagent (ELK Biotechnology, EP013). M-MLV Reverse Transcriptase reagent Kit (ELK Biotechnology, EQ002) was used to synthesize cDNA according to the manufacturer’s instructions. Then, qRT-PCR was performed

(4) Combination of tolvaptan and valsartan improves cardiac and renal functions in doxorubicin-induced heart failure in miceEuropean Journal of HistochemistryNovember 11, 2022Fengqin Yan, Hong Zhu, Yingxia He, Qinqin Wu, Xiaoyu DuanELK Biotechnology). Next, total RNA was transcribed into cDNA using M-MLV Reverse Transcriptase (catalog n. EQ002, ELK Biotechnology). After that, to detect the IL-6 and TNF-a level in heart and kidney tissues, qPCR

(5) VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-ÎºB/ICAM-1 signaling pathwayEuropean Journal of HistochemistryOctober 27, 2022Meiying Zhang, Min Zhou, Xia Cai, Yan Zhou, Xueling Jiang, Yan Luo, Yue Hu, Rong Qiu, Yanrong Wu, Yuejin Zhang, et al.The cDNA was synthesized from the total RNA samples using the M-MLV Reverse Transcriptase Reagent (EQ002, ELK Biotechnology). The cDNA samples were used as a template and amplified using StepOne™ Real-Time PCR System

(6) Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel via targeting miR-200b-3p/Bcl-2 axisSeptember 20, 2022Jindong Li, Jie Kang, Weiyan Liu, Jiazhe Liu, Gaofeng Pan, Anwei Mao, Qing Zhang, Jingfeng Lu, Junbin Ding, Hongchang Lithe purified RNA was reverse-transcribed to form cDNA using a M-MLV Reverse Transcriptase kit (No. EQ002). Next, qPCR experiments were carried out using the QuFast SYBR Green PCR (No. EQ001). All kits were

(7) Characterization of Immune-Related Molecular Subtypes and a Prognostic Signature Correlating With the Response to Immunotherapy in Patients With Gastric CancerFrontiers in ImmunologyJuly 8, 2022Gaoming Wang, Ludi Yang, Yongkun Wang, Renhao Hu, Kehui Zhang, Taohua Guo, Bo Chen, Xiaohua Jiang, Ran Cuiinstructions. Reverse transcription was performed using M-MLV Reverse Transcriptase (ELK Biotechnology, EQ002). RT–qPCR was performed with QuFast SYBR Green PCR Master Mix (ELK Biotechnology, EQ001) and a StepOne™ Real-Time PCR System

(8) Nicotine promotes chronic obstructive pulmonary disease via inducing pyroptosis activation in bronchial epithelial cellsMolecular medicine reportsMarch 1, 2022Mo R, Zhang J, Chen Y, Ding Y.transcribed into cDNA by M-MLV Reverse Transcriptase kit (Elk Wuhan Biotechnology Co., Ltd. cat. no. EQ002) with the following temperature protocol: 42°C for 50 min for the reverse transcription reaction; 99°C

(9) Exosomal microRNA-618 derived from mesenchymal stem cells attenuate the progression of hepatic fibrosis by targeting Smad4BioengineeredMarch 1, 2022Chao Sun, Cuicui Shi, Xiaoyan Duan, Yi Zhang, Baocan WangELK Biotechnology) was used for miRNA reverse transcription. Meanwhile, EntiLink™ 1st Strand cDNA Synthesis kit (EQ002; ELK Biotechnology) was used for mRNA reverse transcription. Remarkably, the specific sequences of primers

(10) Novel Role of miR-18a-5p and Galanin in Rat Lung Ischemia Reperfusion-Mediated ResponseOxidative Medicine and Cellular LongevityAugust 14, 2021Kun Xiao, Lei Song, Ye Hu, Wanxue He, Fei Hou, Peng Yan, Jianqiao Xu, Kaifei Wang, Yi Tao, Dan Li, et al.described in Supplementary Figure 1B. 2.9. First-Strand cDNA Synthesis MMLV RT kit (ELK Biotechnology EQ002) was used for first-strand cDNA synthesis. The manufacturer's protocol was followed. 2.10. Real-Time PCR

(11) Molecular mechanism of extracellular matrix disorder in pelvic organ prolapsesMolecular medicine reportsDecember 1, 2020Zhang L, Dai F, Chen G, Wang Y, Liu S, Zhang L, Xian S, Yuan M, Yang D, Zheng Y, et al.Scientific, Inc.) and reverse transcribed into cDNA using the M‑MLV Reverse Transcriptase kit [cat. no. EQ002; ELK (Wuhan) Biotechnology Co., Ltd.]. The following temperature protocol was used for reverse transcription:

(12) The Optimal Outcome of Suppressing Ewing Sarcoma Growth in vivo With Biocompatible Bioengineered miR-34a-5p ProdrugFrontiers in oncologyJanuary 1, 2020Li DF, Yuan Y, Tu MJ, Hu X, Li YZ, Yi WR, Li PC, Zhao Y, Cheng Z, Yu AM, et al.ELK Biotechnology, Wuhan, China). Reverse transcription was performed using M-MLV Reverse Transcriptase (EQ002; ELK Biotechnology, Wuhan, China) and random hexamers (for U6) or stem-loop primer 5′-CTC AAC TGG TGT

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